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tbst, electrophoretic buffer and electrotransfer solution  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science tbst, electrophoretic buffer and electrotransfer solution
    Tbst, Electrophoretic Buffer And Electrotransfer Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electrotransfer+buffer/tbst++electrophoretic+buffer+and+electrotransfer+solution/pm34295249-54-4-9
    Average 90 stars, based on 1 article reviews
    tbst, electrophoretic buffer and electrotransfer solution - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Electrotransfer:

    Article Title: The Protective Effect of Liquiritin in Hypoxia/Reoxygenation-Induced Disruption on Blood Brain Barrier.
    Article Snippet: SDS-PAGE rapid dispensing kit and ECL chromogenic solution were obtained from EpiZyme Biotechnology (Shanghai, China). .. TBST, electrophoretic buffer and electrotransfer solution were purchased from Beijing Solarbio Technology (Beijing, China). ..

    Article Title: Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.
    Article Snippet: .. The total protein in the tissues of each group was extracted using radio-immunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (P1260 Solarbio, Beijing, China), loaded on 10% or 12% SDS-PAGE gel with the constant current; subsequently, it was transferred to a polyvinylidene difluoride (PVDF) membrane using a wet electrotransfer device (Bio-Rad). .. The following specific primary antibodies were incubated with PVDF membranes overnight at 4 °C: rabbit anti-VEGF receptor 3 (VEGFR3) (1:500, ThermoFisher, PA5-16871), rabbit anti-vascular endothelial (VE)-cadherin (1:500, ThermoFisher, 36-1900), rabbit anti-PROX1 (1:500, Abclonal, A9047), rabbit anti-FOXC2 (1:500, Proteintech, 23066– 1-ap), rabbit anti-VEGFC (1:500, Abclonal, A2556), mouse anti-GAPDH (1:1000, ZSGB-BIO, TA-08), mouse anti-Tau5 (1:1000, Abcam, ab80579), rabbit anti-phospho-tau (Thr205) (1:2000, Abclonal, AP0168), rabbit anti-phospho-tau (Ser404) (1:2000, Abclonal, AP0170), rabbit anti-phospho-tau (Ser396) (1:1000, Cell Signaling Technology, 9632 s).

    Radio Immunoprecipitation:

    Article Title: Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.
    Article Snippet: .. The total protein in the tissues of each group was extracted using radio-immunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (P1260 Solarbio, Beijing, China), loaded on 10% or 12% SDS-PAGE gel with the constant current; subsequently, it was transferred to a polyvinylidene difluoride (PVDF) membrane using a wet electrotransfer device (Bio-Rad). .. The following specific primary antibodies were incubated with PVDF membranes overnight at 4 °C: rabbit anti-VEGF receptor 3 (VEGFR3) (1:500, ThermoFisher, PA5-16871), rabbit anti-vascular endothelial (VE)-cadherin (1:500, ThermoFisher, 36-1900), rabbit anti-PROX1 (1:500, Abclonal, A9047), rabbit anti-FOXC2 (1:500, Proteintech, 23066– 1-ap), rabbit anti-VEGFC (1:500, Abclonal, A2556), mouse anti-GAPDH (1:1000, ZSGB-BIO, TA-08), mouse anti-Tau5 (1:1000, Abcam, ab80579), rabbit anti-phospho-tau (Thr205) (1:2000, Abclonal, AP0168), rabbit anti-phospho-tau (Ser404) (1:2000, Abclonal, AP0170), rabbit anti-phospho-tau (Ser396) (1:1000, Cell Signaling Technology, 9632 s).

    Lysis:

    Article Title: Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.
    Article Snippet: .. The total protein in the tissues of each group was extracted using radio-immunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (P1260 Solarbio, Beijing, China), loaded on 10% or 12% SDS-PAGE gel with the constant current; subsequently, it was transferred to a polyvinylidene difluoride (PVDF) membrane using a wet electrotransfer device (Bio-Rad). .. The following specific primary antibodies were incubated with PVDF membranes overnight at 4 °C: rabbit anti-VEGF receptor 3 (VEGFR3) (1:500, ThermoFisher, PA5-16871), rabbit anti-vascular endothelial (VE)-cadherin (1:500, ThermoFisher, 36-1900), rabbit anti-PROX1 (1:500, Abclonal, A9047), rabbit anti-FOXC2 (1:500, Proteintech, 23066– 1-ap), rabbit anti-VEGFC (1:500, Abclonal, A2556), mouse anti-GAPDH (1:1000, ZSGB-BIO, TA-08), mouse anti-Tau5 (1:1000, Abcam, ab80579), rabbit anti-phospho-tau (Thr205) (1:2000, Abclonal, AP0168), rabbit anti-phospho-tau (Ser404) (1:2000, Abclonal, AP0170), rabbit anti-phospho-tau (Ser396) (1:1000, Cell Signaling Technology, 9632 s).

    SDS Page:

    Article Title: Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.
    Article Snippet: .. The total protein in the tissues of each group was extracted using radio-immunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (P1260 Solarbio, Beijing, China), loaded on 10% or 12% SDS-PAGE gel with the constant current; subsequently, it was transferred to a polyvinylidene difluoride (PVDF) membrane using a wet electrotransfer device (Bio-Rad). .. The following specific primary antibodies were incubated with PVDF membranes overnight at 4 °C: rabbit anti-VEGF receptor 3 (VEGFR3) (1:500, ThermoFisher, PA5-16871), rabbit anti-vascular endothelial (VE)-cadherin (1:500, ThermoFisher, 36-1900), rabbit anti-PROX1 (1:500, Abclonal, A9047), rabbit anti-FOXC2 (1:500, Proteintech, 23066– 1-ap), rabbit anti-VEGFC (1:500, Abclonal, A2556), mouse anti-GAPDH (1:1000, ZSGB-BIO, TA-08), mouse anti-Tau5 (1:1000, Abcam, ab80579), rabbit anti-phospho-tau (Thr205) (1:2000, Abclonal, AP0168), rabbit anti-phospho-tau (Ser404) (1:2000, Abclonal, AP0170), rabbit anti-phospho-tau (Ser396) (1:1000, Cell Signaling Technology, 9632 s).

    Membrane:

    Article Title: Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.
    Article Snippet: .. The total protein in the tissues of each group was extracted using radio-immunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (P1260 Solarbio, Beijing, China), loaded on 10% or 12% SDS-PAGE gel with the constant current; subsequently, it was transferred to a polyvinylidene difluoride (PVDF) membrane using a wet electrotransfer device (Bio-Rad). .. The following specific primary antibodies were incubated with PVDF membranes overnight at 4 °C: rabbit anti-VEGF receptor 3 (VEGFR3) (1:500, ThermoFisher, PA5-16871), rabbit anti-vascular endothelial (VE)-cadherin (1:500, ThermoFisher, 36-1900), rabbit anti-PROX1 (1:500, Abclonal, A9047), rabbit anti-FOXC2 (1:500, Proteintech, 23066– 1-ap), rabbit anti-VEGFC (1:500, Abclonal, A2556), mouse anti-GAPDH (1:1000, ZSGB-BIO, TA-08), mouse anti-Tau5 (1:1000, Abcam, ab80579), rabbit anti-phospho-tau (Thr205) (1:2000, Abclonal, AP0168), rabbit anti-phospho-tau (Ser404) (1:2000, Abclonal, AP0170), rabbit anti-phospho-tau (Ser396) (1:1000, Cell Signaling Technology, 9632 s).



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