Electrotransfer:
Article Title: Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.
Article Snippet: .. The total protein in the tissues of each group was extracted using radio-immunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (P1260 Solarbio, Beijing, China), loaded on 10% or 12% SDS-PAGE gel with the constant current; subsequently, it was transferred to a polyvinylidene difluoride (PVDF) membrane using a wet electrotransfer device (Bio-Rad). .. The following specific primary antibodies were incubated with PVDF membranes overnight at 4 °C: rabbit anti-VEGF receptor 3 (VEGFR3) (1:500, ThermoFisher, PA5-16871), rabbit anti-vascular endothelial (VE)-cadherin (1:500, ThermoFisher, 36-1900), rabbit anti-PROX1 (1:500, Abclonal, A9047), rabbit anti-FOXC2 (1:500, Proteintech, 23066– 1-ap), rabbit anti-VEGFC (1:500, Abclonal, A2556), mouse anti-GAPDH (1:1000, ZSGB-BIO, TA-08), mouse anti-Tau5 (1:1000, Abcam, ab80579), rabbit anti-phospho-tau (Thr205) (1:2000, Abclonal, AP0168), rabbit anti-phospho-tau (Ser404) (1:2000, Abclonal, AP0170), rabbit anti-phospho-tau (Ser396) (1:1000, Cell Signaling Technology, 9632 s).
Radio Immunoprecipitation:Article Title: Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.
Article Snippet: .. The total protein in the tissues of each group was extracted using radio-immunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (P1260 Solarbio, Beijing, China), loaded on 10% or 12% SDS-PAGE gel with the constant current; subsequently, it was transferred to a polyvinylidene difluoride (PVDF) membrane using a wet electrotransfer device (Bio-Rad). .. The following specific primary antibodies were incubated with PVDF membranes overnight at 4 °C: rabbit anti-VEGF receptor 3 (VEGFR3) (1:500, ThermoFisher, PA5-16871), rabbit anti-vascular endothelial (VE)-cadherin (1:500, ThermoFisher, 36-1900), rabbit anti-PROX1 (1:500, Abclonal, A9047), rabbit anti-FOXC2 (1:500, Proteintech, 23066– 1-ap), rabbit anti-VEGFC (1:500, Abclonal, A2556), mouse anti-GAPDH (1:1000, ZSGB-BIO, TA-08), mouse anti-Tau5 (1:1000, Abcam, ab80579), rabbit anti-phospho-tau (Thr205) (1:2000, Abclonal, AP0168), rabbit anti-phospho-tau (Ser404) (1:2000, Abclonal, AP0170), rabbit anti-phospho-tau (Ser396) (1:1000, Cell Signaling Technology, 9632 s).
Lysis:Article Title: Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.
Article Snippet: .. The total protein in the tissues of each group was extracted using radio-immunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (P1260 Solarbio, Beijing, China), loaded on 10% or 12% SDS-PAGE gel with the constant current; subsequently, it was transferred to a polyvinylidene difluoride (PVDF) membrane using a wet electrotransfer device (Bio-Rad). .. The following specific primary antibodies were incubated with PVDF membranes overnight at 4 °C: rabbit anti-VEGF receptor 3 (VEGFR3) (1:500, ThermoFisher, PA5-16871), rabbit anti-vascular endothelial (VE)-cadherin (1:500, ThermoFisher, 36-1900), rabbit anti-PROX1 (1:500, Abclonal, A9047), rabbit anti-FOXC2 (1:500, Proteintech, 23066– 1-ap), rabbit anti-VEGFC (1:500, Abclonal, A2556), mouse anti-GAPDH (1:1000, ZSGB-BIO, TA-08), mouse anti-Tau5 (1:1000, Abcam, ab80579), rabbit anti-phospho-tau (Thr205) (1:2000, Abclonal, AP0168), rabbit anti-phospho-tau (Ser404) (1:2000, Abclonal, AP0170), rabbit anti-phospho-tau (Ser396) (1:1000, Cell Signaling Technology, 9632 s).
SDS Page:Article Title: Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.
Article Snippet: .. The total protein in the tissues of each group was extracted using radio-immunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (P1260 Solarbio, Beijing, China), loaded on 10% or 12% SDS-PAGE gel with the constant current; subsequently, it was transferred to a polyvinylidene difluoride (PVDF) membrane using a wet electrotransfer device (Bio-Rad). .. The following specific primary antibodies were incubated with PVDF membranes overnight at 4 °C: rabbit anti-VEGF receptor 3 (VEGFR3) (1:500, ThermoFisher, PA5-16871), rabbit anti-vascular endothelial (VE)-cadherin (1:500, ThermoFisher, 36-1900), rabbit anti-PROX1 (1:500, Abclonal, A9047), rabbit anti-FOXC2 (1:500, Proteintech, 23066– 1-ap), rabbit anti-VEGFC (1:500, Abclonal, A2556), mouse anti-GAPDH (1:1000, ZSGB-BIO, TA-08), mouse anti-Tau5 (1:1000, Abcam, ab80579), rabbit anti-phospho-tau (Thr205) (1:2000, Abclonal, AP0168), rabbit anti-phospho-tau (Ser404) (1:2000, Abclonal, AP0170), rabbit anti-phospho-tau (Ser396) (1:1000, Cell Signaling Technology, 9632 s).
Membrane:Article Title: Exogenous interleukin 33 enhances the brain's lymphatic drainage and toxic protein clearance in acute traumatic brain injury mice.
Article Snippet: .. The total protein in the tissues of each group was extracted using radio-immunoprecipitation assay lysis buffer containing protease and phosphatase inhibitors (P1260 Solarbio, Beijing, China), loaded on 10% or 12% SDS-PAGE gel with the constant current; subsequently, it was transferred to a polyvinylidene difluoride (PVDF) membrane using a wet electrotransfer device (Bio-Rad). .. The following specific primary antibodies were incubated with PVDF membranes overnight at 4 °C: rabbit anti-VEGF receptor 3 (VEGFR3) (1:500, ThermoFisher, PA5-16871), rabbit anti-vascular endothelial (VE)-cadherin (1:500, ThermoFisher, 36-1900), rabbit anti-PROX1 (1:500, Abclonal, A9047), rabbit anti-FOXC2 (1:500, Proteintech, 23066– 1-ap), rabbit anti-VEGFC (1:500, Abclonal, A2556), mouse anti-GAPDH (1:1000, ZSGB-BIO, TA-08), mouse anti-Tau5 (1:1000, Abcam, ab80579), rabbit anti-phospho-tau (Thr205) (1:2000, Abclonal, AP0168), rabbit anti-phospho-tau (Ser404) (1:2000, Abclonal, AP0170), rabbit anti-phospho-tau (Ser396) (1:1000, Cell Signaling Technology, 9632 s).
|